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ntdC [2019-07-31 08:07:17]
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ntdC [2019-07-31 08:07:17]

NAD-dependent glucose-6-phosphate dehydrogenase
locus
BSU_10530
pI
6.23
mw
39.14 kDa
protein length
350 aa Sequence Blast
gene length
1053 bp Sequence Blast
function
synthesis of the antibiotic kanosamine
product
NAD-dependent glucose-6-phosphate dehydrogenase
essential
no
synonyms
yhjJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

    • Gene

    Coordinates
    1,126,400 1,127,452

    The protein

    Catalyzed reaction/ biological activity

  • glucose-6-phosphate + NAD - 3-oxo-d-glucose-6-phosphate + NADH(2) [Pubmed|23586652]

    • Protein family

  • [SW|Gfo/Idh/MocA family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|484D17480DFAD3667E0EBC6D38854A7546A8DB46|YteT], [protein|7DFE74C751A67CCC84579D52005E1399B0A10166|IolW], [protein|920C5750CB95102B073C32874D512E8D636D2C7D|IolU], [protein|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|IolG], [protein|942BAE9FA023DFCA06B6D26A856A72F2979E23FA|YrbE], [protein|AA7DCEFB17FD6F9C4F65235E9A247A6EC2242B06|IolX], [protein|03BE6DA854612B3F6741E0E167AB310F0DE96285|YfiI]

    • [SW|Cofactors]

  • NAD+ [Pubmed|23586652]

    • Structure

  • [PDB|3EUW] (from Corynebacterium glutamicum, 21% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA]-[gene|A4DE6C394B64813A732255734758403241FFAC2F|ntdB]-[gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]-[gene|ADB476B0B385D9B9CAF02902CE115C8309F67911|glcP]
    description
    [Pubmed|17056753]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|14612444], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|NtdR]: activation, in [regulon|9118A36CD30E7C99697B3659BB04E03BB7EE71D7|NtdR regulon]

    • regulation

  • induced by 3,3'-neotrehalosadiamine ([protein|search|NtdR]) [Pubmed|14612444]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B286 (yhjJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1285 NBRP B. subtilis, Japan]
  • BKE10530 ([gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTATTTCCTCCTCAT, downstream forward: _UP4_TAGTATTTCAAAGAGAGAAG
  • BKK10530 ([gene|29DAF5343A42EAD8DB97925FE7D049410F7B7FCA|ntdC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTATTTCCTCCTCAT, downstream forward: _UP4_TAGTATTTCAAAGAGAGAAG

    • References

    Reviews

  • 21512256

    • Original publications

  • 19087206,17056753,14612444,23586652,28353336